Sustained efficacy of collars containing 10% w/w imidacloprid and 4.5% w/w flumethrin (Seresto®) in dogs against laboratory challenge with Haemaphysalis longicornis (Neumann, 1901) ticks

Table 1 Arithmetic mean live Haemaphysalis longicornis counts and ranges for control dogs and dogs treated with Seresto^® and percent efficacy relative to control at 48 h after infestations

Day	Control				Seresto^®				Efficacy compared to control
Day	Group	Mean	Range	Dogs with ticks	Group	Mean	Range	Dogs with ticks	%Efficacy^a	Test statistic
3	3	28.6	14–43	8/8	2	3.4	0–7	5/8	88.2	t₍₁₄₎ = 6.47, P < 0.0001
9	3	38.8	30–50	8/8	2	0.1	0–1	1/8	99.7	t₍₁₄₎ = 18.41, P < 0.0001
30	3	36.9	27–45	8/8	2	0.1	0–1	1/8	99.7	t₍₁₄₎ = 20.18, P < 0.0001
58	3	29.3	17–37	8/8	2	0	0–0	0/8	100	t₍₁₄₎ = 11.74, P < 0.0001
94	3	28.6	14–43	8/8	1	0.5	0–2	3/8	98.3	t₍₁₄₎ = 7.56, P < 0.0001
121	3	36.9	27–45	8/8	1	0	0–0	0/8	100	t₍₁₄₎ = 20.29, P < 0.0001
149	3	29.3	17–37	8/8	1	0	0–0	0/8	100	t₍₁₄₎ = 11.74, P < 0.0001
170	3	27.3	19–35	8/8	1	0	0–0	0/8	100	t₍₁₄₎ = 13.46, P < 0.0001
198	3	32.5	25–40	8/8	1	0	0–0	0/8	100	t₍₁₄₎ = 16.95, P < 0.0001
229	3	17.4	7–40	8/8^b	1	0	0–0	0/8	100	t₍₁₄₎ = 4.59, P = 0.0004
240	3	32.5	15–45	8/8	1	0.1	0–1	1/8	99.6	t₍₁₄₎ = 9.97, P < 0.0001

^aPairwise comparison of tick counts between control and treated groups statistically significantly different at all time points (P < 0.001)
^bOn day 229, two out of eight dogs presented with < 13 ticks (7 and 8 ticks, respectively)

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